The role of molecular and genetic methods to determine theТ-cell and В-cell clonality in diagnosing malignant cutaneouslymphomas

Abstract


The article presents the data on the studies contributing to improving the differential diagnostics of T-cell and B-cell
cutaneous lymphomas including large-plaque parapsoriasis and T-cell and B-cell cutaneous pseudolymphomas as well
as frequency of their transformation into malignant cutaneous lymphomas. There was a study of 101 patients using the
polymerase chain reaction method to determine the T-cell and B-cell lymphocyte clonality by genes of g and ƒ chains in
the T-cell receptor and immunoglobulin heavy chain genes. Monoclonality was determined in 40 of 46 cases in patients
with T-cell cutaneous lymphomas and in three of four cases in patients with B-cell cutaneous lymphomas. Monoclonality
was revealed in one of 14 cases of large-plaque parapsoriasis and one of two cases of T-cell cutaneous pseudolymphoma.
In all of the 24 cases of chronic benign dermatoses, five cases of small-plaque parapsoriasis and ten skin tissue samples
obtained from healthy donors, polyclonal lymphocytes were revealed. So, the obtained results make it possible to
consider the method to be an important addition in the field of diagnosing lymphoproliferative skin diseases.

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Copyright (c) 2011 Molochkov V.A., Sidorova Y.V., Groznova A.A., Ovsyannikova G.V., Fedorovskaya A.V., Molochkov A.V., Sudarikov A.B., Molochkov A.V., Sidorova Y.V., Groznova A.A., Ovsyannikova G.V., Fedorovskaya A.V., Molochkov A.V., Sudarikov A.B.

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