Modern immunochromatographic methods for determination of anti-treponema pallidum antigenantibodies

Cite item


The authors present a review of research publications about clinical significance of using modern
immunochromatographic methods for determination of anti-immunodominant antigen antibodies of syphilis pathogen
Treponema pallidum.
Immunochromatographic sets of chemicals proved to be convenient in use, can be used with a small amount of biological
materials, do not demand any specialized medical equipment, have high sensitivity and specificity, in particular, for
tests with blood serum or plasma samples; when whole blood samples are used, this reduces the method sensitivity.
Immunochromatographic tests are intended for prompt express examination of the population to reveal syphilis patients
under field conditions or at medical institutions consulting primary patients and not having their own clinical laboratories.


  1. Branson B.M. Point-of-care raped test for HIV antibodies. J Lab Med 2003; 27: 288-295.
  2. Hurt A., Barr I. Raped diagnostic kits for influenza. Microb Australia 2006 (May): 31-63.
  3. Rosen S. Market Trends in Lateral Flow Immunoassay. In: Wong R.C. (ed.) et Tse H.Y. (ed.) Lateral Flow Immunoassay. New-York: Humana Press, 2009: 35-50.
  4. Malaria Rapid Diagnostic Test Performance. Results of WHO product testing of malaria RDTs: Round 2 (2009) ( 2010/9789241599467_eng.pdf).
  5. Akyar I., Kocagoz T., Sinik G. et al. Lateral flow assey foe rapid differentiation of Mycobacterium tuberculosis and species of mycobacterium other then tuberculosis grown in Löwenstein- Jensen medium. Indian J Med Microbiol 2010 (Oct-Dec); 28 (4): 308-312.
  6. Campbell R.L., Wagner D.B., O'Connel J.P. Solid-phase assay with visual readout. US Pat. 4.703.017; 1987.
  7. Rosenstain R.W., Bloomster T.G. Solid-phase assay employing capillary flow. US Pat. 4.855.240; 1989.
  8. May K., Prior M.E., Richard I. Capillary assay and device therefore comprising mobilizable particulate labeled reagents. US Pat. 5.622.871; 1997.
  9. O'Farrell B.O. Evolution in Lateral Flow-Based Immunoassay Systems. In: Wong R.C. (ed.) et Tse H.Y. (ed.) Lateral Flow Immunoassay. New- York: Humana Press, 2009: 1-34.
  10. Ponti J.S. Material Platform for the Assembly of Lateral Flow Immunoassay Strips. In: Wong R.C. (ed.) et Tse H.Y. (ed.) Lateral Flow Immunoassay. New-York: Humana Press, 2009: 49-34.
  11. Денисова О.В., Тогузов Р.Т. Значение и перспективы использования методов бесприборной диагностики в клинической лабораторной практике. Клин. лаб. диагн. 2001; 9: 42.
  12. Jiang T., Liang Z., Ren W. et al. Development and validation of a lateral flow imunoassey using colloidal gold for identification of serotypespecific foot-and-mouth desease virus O, A and Asia 1. J Virol Methods 2001 (Jan); 171 (1): 74-80.
  13. Alveres I., Gutierres G., Barrandeguy M., Trono K. Immunochromotographic lateral flow test for detection of antibodies Infectious anemia virus. J Virol Methods 2010 (Aug); 167 (2): 152-157.
  14. Федосова Н.Ю., Вяткина Т.Г., Баранова С.Г. «Сифилис-экспресс» - тест-система для быстрого выявления антител к Treponema pallidum в сыворотке, плазме и цельной крови // IX Всероссийский съезд дерматовене- рологов. М, 2005: 51.
  15. Peeling R.W., Holmes K.K. et al. Rapid tests for sexually transmitted infections (STDs): the way forward. Sex Transm Infect 2006 (Dec); 82 (5): 1-6.
  16. Chum P. Colloidal Gold and Other Labels for Lateral Flow Immunoassays. In: Wong R.C. (ed.) et Tse H.Y. (ed.) Lateral Flow Immunoassay. New-York: Humana Press 2009: 75-93.
  17. Mansfield M.A. Nitrocellulose Membranes for Lateral Flow Immunoassays. In: Wong R.C. (ed.) et Tse H.Y. (ed.) Lateral Flow Immunoassay. New-York: Humana Press 2009: 94-114.
  18. Ротанов С.В., Фриго Н.В., Клюева В.И. Срав- нительное изучение иммунохроматографических наборов для экспресс-диагностики сифилиса. Клин. лаб. диагн. 2008: 2: 42-45.
  19. Determine™ Syphilis TP (www.determinetest. com/news).
  20. McGill M.A., Edmondson D.G., Carroll J.A. et al. Characterization and serological analyses of the Treponema pallidum proteome. Infect Immun 2010 (Jun); 78 (6): 2631-43.
  21. Seňa A.C., White B.L., Sparling P.E. Novel Treponema pallidum serologic tests: a paradigm shift in syphilis screening for 21st century. Clin Infect Dis 2010 (Sep); 51 (6): 700-708.
  22. Чепурченко Н.В., Гладышева М.В., Обрядина А.П. Новые возможности использования рекомбинантных антигенов в серодиагностике сифилиса. Клин. дерматол. и венерол. 2006; 6: 28-31.
  23. Kevin J. A New Platform For Lateral Flow Immunoassay Tests. In: Wong R.C. (ed.) et Tse H.Y. (ed.) Lateral Flow Immunoassay. New-York: Humana Press 2009: 115-129.
  24. Radebe F.M., Khumalo S., Tshabalala V. et al. Evaluation of two rapid tests for the detection of antibodies to syphilis. Lesedi Africa'99, IUSTI/STD/HIV 6th World Congress & 38th IUSTI General Assambly. 21-24 Nov 1999, Sun City. Abstract book: STD 12. (цит.: ИППП 2000; 1: 52).
  25. Campos P.E., Buffardi A.L., Chiappe M. et al. Utility of the Determine Syphilis TP rapid test in commercial sex venues in Peru. Sex Transm Infect. 2006 (Dec); 82 (5): 22-5.
  26. Benzaken A.S., Sabido M., Galban E.G. et al. Field evaluation of the performance and testing cost of a rapid point-of care test for syphilis in red-light district of Manaus, Brazil. Sex Transm Inf 2008; 84: 297-302.
  27. Nessa K., Alam A., Chawdhury F.A. et al. Field evaluation of simple rapid tests in the diagnosis of syphilis. Int J STD AIDS 2008 (May); 19(5): 316-20.
  28. Montoya P.J., S.A.Lukehart, P.E. Brentinger et al. Comparison of the diagnostic accuracy of a rapid immunochromatographic & rapid plasma regain test for antenatal syphilis screening in Mozambique. Bull WHO 2006 (Feb); 84 (2): 97-104.
  29. Angue Y., Yauieb A., Mola G. et al. Syphilis serology testing: a comparative study of Abbot Determine, Rapid Plasma Reagin (RPR) card test and Venereal Disease Research Laboratory (VDRL) methods. P N G Med J 2005 (Sep-Dec); 48 (3-4): 168-173.
  30. Tinajeros F., Grossman D., Richmond K. et al. Diagnostic accuracy of a point-of-care syphilis test when used among pregnant women in Bolivia. Sex Transm Inf 2006 (Dec); 82 (5): 17-21.
  31. Wang L.N., Zheng H.Y., Li J. et al. [Sensitivity and specificity of ELISA based on recombinant T.pallidum rapid regain test in diagnosis of syphilis: a comparative study]. Zhonghua Yi Xue Za Zhi 2007 (Jun); 87(24): 1721-2.
  32. van Dommelen L., Smismans A., Goosens V.J. et al. Evaluation of a rapid one-step immunochromatographic test and two immunoenzymatic assays for the detection of anti-Treponema pallidum antibodies. Sex Transm Inf 2008; 84: 292-296.
  33. Lin L.R., Fu Z.G., Dan B. et al. Development of a colloidal gold-immunochromatography assay to detect immunoglobulin G antibodies to Treponema pallidum with TpN17 и TpN47. Diagn Microbiol Infect Dis 2010 (Nov); 68 (3): 193-200.
  34. Peeling R.W., Ye H. Diagnostic tools for preventing and managing maternal and congenital syphilis: an overview. Bull World Health Organ 2004 (Jun); 82 (6): 439-46.
  35. Laboratory-based evaluation of rapid syphilis diagnostics. Results from 8 SDI Sites. UNDP/ World Bank/WHO; The Sexually Transmitted Diseases Diagnostics Initiative (SDI); Special Programme for Research and Training in Tropical Diseases (TDR): Geneva 2003; 32.
  36. Herring A. J., Ballard R.C., Pope V. et al. A multicentre evaluation of nine rapid, point-of-care syphilis tests using archived sera. Sex Transm Infect 2006;V: 7-12 (www.

Copyright (c) 2011 Rotanov S.V., Osmanova S.R., Rotanov S.V., Osmanova S.R.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 60448 от 30.12.2014.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies