Vestnik dermatologii i venerologiiVestnik dermatologii i venerologiiВестник дерматологии и венерологии0042-46092313-6294Rossijskoe Obschestvo Dermatovenerologov i Kosmetologov120210.25208/vdv1202ORIGINAL STUDIESНАУЧНЫЕ ИССЛЕДОВАНИЯResearch ArticleSkin cytokine profile in patients with mycosis fungoidesЦитокиновый профиль кожи у больных грибовидным микозомhttps://orcid.org/0000-0003-3805-84893604-6491KaramovaArfenya E.КарамоваАрфеня Эдуардовна
Russian Federation

MD, Cand. Sci. (Med.)

к.м.н.

karamova@cnikvi.ruhttps://orcid.org/0000-0001-7214-81763859-7081NikonorovAlexandr А.НиконоровАлександр Александрович
Russian Federation

MD, Dr. Sci. (Med.), Professor

д.м.н.

nikonorov_all@mail.ruhttps://orcid.org/0000-0002-1104-76948261-6561VerbenkoDmitry A.ВербенкоДмитрий Анатольевич
Russian Federation

Cand. Sci. (Biol.)

к.б.н.

verbenko@gmail.comhttps://orcid.org/0000-0002-2553-04849552-7850ZnamenskayaLyudmila F.ЗнаменскаяЛюдмила Федоровна
Russian Federation

MD, Dr. Sci. (Med.)

д.м.н.

znaml@cnikvi.ruhttps://orcid.org/0000-0002-3129-00508334-2890VorontsovaAnastasiiaВоронцоваАнастасия Александровна
Russian Federation

junior research associate

младший научный сотрудник

vorontsova@cnikvi.ruState Research Center of Dermatovenereology and Cosmetology, Ministry of Health of the Russian FederationФГБУ «Государственный научный центр дерматовенерологии и косметологии» Минздрава России1512202096614192812202011012021Copyright ©; 2020, Karamova A.E., Nikonorov A.А., Verbenko D.A., Znamenskaya L.F., Vorontsova A.Copyright ©; 2020, Карамова А.Э., Никоноров А.А., Вербенко Д.А., Знаменская Л.Ф., Воронцова А.А.2020Karamova A.E., Nikonorov A.А., Verbenko D.A., Znamenskaya L.F., Vorontsova A.Карамова А.Э., Никоноров А.А., Вербенко Д.А., Знаменская Л.Ф., Воронцова А.А.https://creativecommons.org/licenses/by/4.0https://vestnikdv.ru/jour/article/view/1202

Aim of the study. To determine the concentration of cytokines in the skin of patients with mycosis fungoides and correlations between their concentration and the value of the modified scale for assessing the severity of skin lesions mSWAT.

Methods. The concentration of cytokines was simultaneously determined using xMAP technology: IL-1â, IL-4, IL-6, IL-10, IL-17A, IL-17F, IL-21, IL-22, IL-23, IL-25, IL -31, IL-33, IFN-ã, sCD40L, TNF-á in skin biopsies of 21 patients with early (IA—IIA) stages of mycosis fungoides and 4 healthy individuals. Analysis and visualization of the obtained data were carried out using R Statistical Software for MacOS (version 1.3.1056), a free open source software development environment for the R programming language.

Results. An increase in the level of IL-4 and TNF-á in the lesions in patients with mycosis fungoides compared with healthy individuals was shown (p = 0.025 and p = 0.012, respectively). Correlation analysis revealed the formation of cytokine networks in the skin of patients with mycosis fungoides that were not associated with the value of the modified scale for assessing the severity of skin lesions mSWAT. A tendency towards a negative relationship between the mSWAT and IFNã scales was found (p = 0.056).

Conclusion. None of the cytokines in lesions and visually unaffected skin from patients with mycosis fungoides were associated with mSWAT values.

Цель исследования: определить концентрации цитокинов в коже больных грибовидным микозом и оценить взаимосвязи со значением модифицированной шкалы оценки тяжести кожного поражения mSWAT.

Методы: с использованием технологии xMAP одновременно определяли концентрацию цитокинов: IL-1β, IL-4, IL-6, IL-10, IL-17A, IL-17F, IL-21, IL-22, IL-23, IL-25, IL-31, IL-33, IFN-γ, sCD40L, TNF-α. в биоптатах кожи 21 больного ранними (IA-IIA) стадиями грибовидного микоза и 4 здоровых лиц. Анализ и визуализация полученных данных проведены с использованием R Statistical Software for MacOS (версия 1.3.1056), свободная среда разработки программного обеспечения с открытым исходным кодом для языка программирования R.

Результаты: показано повышение уровня IL4 и TNFa в очагах поражения у больных ГМ по сравнению со здоровыми лицами (р= 0.025 и р= 0.012, соотвественно). С помощью корреляционного анализа выявлено формирование в коже больных ГМ цитокиновых сетей, не связанных со значением модифицированной шкалы оценки тяжести кожного поражения mSWAT. Обнаружена тенденция к отрицательной взаимосвязи шкалы mSWAT и IFNg (р=0.056)

Заключение: ни один из цитокинов в очагах поражения и визуально непораженной коже не был связан с величиной mSWAT.

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