Vestnik dermatologii i venerologiiVestnik dermatologii i venerologiiВестник дерматологии и венерологии0042-46092313-6294Rossijskoe Obschestvo Dermatovenerologov i Kosmetologov310.25208/0042-4609-2014-90-1-17-24SHORT COMMUNICATIONКРАТКИЕ СООБЩЕНИЯApplication of Confocal Laser Scanning Microscopy in Biology and MedicineПрименение конфокальной лазерной сканирующей микроскопии в биологии и медицинеVolkovI. A.ВолковИ. А.
Russian Federation
ivolkov@cnikvi.ruFrigoN. V.ФригоН. В.
Russian Federation
ZnamenskayaL. F.ЗнаменскаяЛ. Ф.
Russian Federation
KatuninaO. R.КатунинаО. Р.
Russian Federation
State Research Center of Dermatovenereology and Cosmetology, Ministry of Healthcare of the Russian FederationФГБУ «Государственный научный центр дерматовенерологии и косметологии» Минздрава России24022014901172424082017Copyright ©; 2014, Volkov I.A., Frigo N.V., Znamenskaya L.F., Katunina O.R.Copyright ©; 2014, Волков И.А., Фриго Н.В., Знаменская Л.Ф., Катунина О.Р.2014Volkov I.A., Frigo N.V., Znamenskaya L.F., Katunina O.R.Волков И.А., Фриго Н.В., Знаменская Л.Ф., Катунина О.Р.https://creativecommons.org/licenses/by/4.0https://vestnikdv.ru/jour/article/view/3

Fluorescence confocal laser scanning microscopy and reflectance confocal laser scanning microscopy are up-to-date highend study methods. Confocal microscopy is used in cell biology and medicine. By using confocal microscopy, it is possible to study bioplasts and localization of protein molecules and other compounds relative to cell or tissue structures, and to monitor dynamic cell processes. Confocal microscopes enable layer-by-layer scanning of test items to create demonstrable 3D models. As compared to usual fluorescent microscopes, confocal microscopes are characterized by a higher contrast ratio and image definition.

Методы флуоресцентной и отражательной конфокальной лазерной сканирующей микроскопии являются современными высокотехнологичными методами исследования. Конфокальная микроскопия находит практическое применение в клеточной биологии и медицине. С помощью конфокальной микроскопии возможно осуществить изучение клеточных органелл и локализации молекул белков и других соединений относительно структур клетки или ткани, а также провести наблюдение за динамическими процессами в клетке. Конфокальные микроскопы позволяют проводить послойное сканирование исследуемых препаратов. Это дает возможность создавать наглядные объемные 3D модели. По сравнению с обычными флуоресцентными микроскопами конфокальные микроскопы обладают повышенной контрастностью и четкостью изображения.

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