Potential of confocal laser scanning microscopy for non-invasive diagnostics of malignant epithelial skin tumors in the course of dermatoheliosis progression

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Most cases of malignant epithelial skin neoplasms including actinic keratosis and basal cell carcinoma, which are characterized by the most complicated course and numerous clinical and morphological options, involve dermatoheliosis progression. The risk of actinic keratosis transformation into basal cell carcinoma varies from 0.1% to 20% and up to 80% in cases of multiple AK lesion foci. A non-invasive method known as reflectance confocal laser scanning microscopy is the most promising one for the purposes of early diagnostics of signs pointing at epithelial skin neoplasm development and makes it possible to monitor the tumor in progress in vivo to diagnose the presence of a pool of squamous cells on a timely basis. The confocal laser scanning microscopy method provides high-contrast images of for any horizontal-oriented morphologic structures in the epidermis and upper dermis with a resolution comparable to those characteristic of traditional optical microscopy of skin tissue samples. According to our data obtained as a result of studying dynamic changes and morphologic structures in actinic keratosis foci (50 cases) using the confocal laser scanning microscopy method, we discovered a number of morphologic features, and their further analysis will distinguish the signs of progressing carcinogenesis in case of dermatoheliosis.

About the authors

E. S. Snarskaya

I.M. Sechenov First Moscow State Medical University

Author for correspondence.
Email: snarskaya-dok@mail.ru
Russian Federation

S. B. Tkachenko

I.M. Sechenov First Moscow State Medical University

Email: noemail@neicon.ru
Russian Federation

E. V. Kuznetsova

I.M. Sechenov First Moscow State Medical University

Email: noemail@neicon.ru
Russian Federation

A. S. Allenova

I.M. Sechenov First Moscow State Medical University

Email: noemail@neicon.ru
Russian Federation


  1. Снарская Е.С. Фотостарение кожи: современные аспекты. Вестн дерматол венерол 2011; (2): 98-103
  2. Fisher G.J., Kang S., Varani J. et al. Mechanisms of photoaging and chronological skin aging. Arch Dermatol 2002 Nov; 138 (11): 1462-70.
  3. Wu S., Han J., Vlegus R.A. et al. Cumulative ultraviolet radiation flux in adulthood and risk of incident skin cancers in women. Br J Cancer. 2014 Apr 2; 110 (7): 1855-61.
  4. Lesiak A., Slowik-Rylska M., Rogowski-Tylman M. et al. Risk factors in Central Poland for the development of superficial and nodular basal cell carcinomas. Arch Med Sci 2010 Apr 30; 6 (2): 270-5.
  5. Memon A.A., Tomenson J.A., Bothwell J. et al. Prevalence of solar damage and actinic keratosis in a Merseyside population. Br J Dermatol 2000; 142: 1154-9.
  6. Schaefer I., Augustin M., Spehr C. et al. Prevalence and risk factors of actinic keratoses in Germany - analysis of multisource data. J Eur Acad Dermatol Venereol 2013; 28: 309-13.
  7. Traianou A., Ulrich M., Apalla Z. et al. Risk factors for actinic keratosis in eight European centers:a case-control study. Br J Dermatol 2012; 167 (Suppl 2): 36-42.
  8. Callen J.P., Bickers D.R., Moy R.L. Actinic keratoses. J Am Acad Dermatol 1997 Apr; 36 (4): 650-653.
  9. Drake L.A., Ceilley R.I., Cornelison R.L., Dobes W.L., Dorner W., Goltz R.W., et al. Guidelines for care of actinic keratoses. J Am Acad Dermatol 1995; 32: 95-8.
  10. Babilas P., Landthaler M., Szeimies R.M. Actinic keratoses. Hautarzt 2003; 54: 551-62.
  11. Röwert-Huber J1, Patel MJ, Forschner T, Ulrich C, Eberle J, Kerl H, Sterry W, Stockfleth E. Actinic keratosis is an early in situ squamous cell carcinoma: a proposal for reclassification. Br J Dermatol. 2007 May; 156 Suppl 3: 8-12
  12. Strunk T., Braathen L.R., Szeimies R.-M. Актинический кератоз - обзор литературы. Вестн дерматол венерол 2014; (5): 42-52
  13. Zalaudek I, Giacomel J, Schmid K, et al. Dermatoscopy of facial actinic keratosis, intraepidermal carcinoma, and invasive squamous cell carcinoma: a progression model. J Am Acad Dermatol 2012 Apr; 66 (4): 589-97.
  14. Traianou A, Ulrich M, Apalla Z, et al. Risk factors for actinic keratosis in eight European centres: a case-control study. Br J Dermatol 2012 Aug; 167 Suppl 2: 36-42.
  15. Khalesi M, Whiteman DC, Doi SA, et al. Cutaneous markers of photo-damage and risk of Basal cell carcinoma of the skin: a meta-analysis. Cancer Epidemiol Biomarkers Prev 2013 Sep; 22 (9): 1483-9.
  16. Снарская Е.С., молочков В.А., Пылев Л.Н. и др. Экспериментальный метатипический рак кожи (первая отечественная модель). Рос журн кожн венерич болезней 2004; (6): 66-70
  17. Снарская Е.С., молочков В.А. Базалиома. м 2003: 136
  18. Кондратьева Е.Г., Ламоткин И.А. Ошибки в диагностике базально-клеточного рака кожи. 2009; (3): 71
  19. Лукашева Н.Н., Ткаченко С.Б., Потекаев Н.Н. и др. Прижизненная отражательная конфокальная лазерная сканирующая микроскопия: история создания, принцип работы, возможности применения в дерматологии. Клин дерматол венерол 2008; (5):10-15
  20. Peppelman M., Nguyen K.P., Hoogedoorn L. et al. Reflectance confocal microscopy: non-invasive distinction between actinic keratosis and squamous cell carcinoma. J Eur Acad Dermatol Venereol. 2015 Jul; 29 (7): 1302-9
  21. Ulrich M., Maltusch A., Rius-Diaz F. et al. Clinical applicability of in vivo reflectance confocal microscopy for the diagnosis of actinic keratoses. Dermatol Surg 2008 May; 34 (5): 610-9.
  22. Horn M., Gerger A., Ahlgrimm-Siess V. et al. Discrimination of actinic keratoses from normal skin with reflectance mode confocal microscopy. Dermatol Surg 2008 May; 34 (5): 620-5.
  23. Rishpon A., Kim N., Scope A. et al. Reflectance confocal microscopy criteria for squamous cell carcinomas and actinic keratoses. Arch Dermatol 2009 Jul; 145 (7): 766-72.

Copyright (c) 2016 Snarskaya E.S., Tkachenko S.B., Kuznetsova E.V., Allenova A.S.

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