Optical coherence tomography and polarimetry of superficial skin biopsies

Cover Page


Cite item

Full Text

Abstract

The goal. Of this study was to develop and assess the efficacy of polarization probing of biotissues in vitro. The method is based on the determination of polarization parameters of scattered radiation. Materials and methods. The well-known superficial epidermis stripping method was applied using the Sulfacrylate self-sterile medical adhesive. Small portions of thin layers of the adhesive were applied to slide plates and then to different skin sites. The corneous layer in the normal condition and in case of skin diseases (psoriasis, lichen acuminatus, discoid lupus erythematosus, alopecia, itching and demodectic mange) was examined based on the optical coherence tomography (OCT) method using the 0CS1300SS device (Thorlabs Inc, USA). Results. The authors obtained pictures visualizing the structural organization of different layers of the epidermis using the superficial epidermis biopsy method in case of lichen acuminatus, hyperkeratosis, itching and other skin diseases. Conclusion. This method ensures non-invasive high-precision measurement of the structure of different layers of the epidermis, which may be useful both for research purposes and practical dermatology.

About the authors

S. R. Utz

Saratov State Medical University named after V.I. Razumovsky

Author for correspondence.
Email: kalinina13@mail.ru
Russian Federation

D. A. Zimnyakov

Yuri Gagarin State Technical University of Saratov

Email: kalinina13@mail.ru
Russian Federation

E. M. Galkina

Saratov State Medical University named after V.I. Razumovsky

Email: kalinina13@mail.ru
Russian Federation

E. M. Reshetnikova

Saratov State Medical University named after V.I. Razumovsky

Email: kalinina13@mail.ru
Russian Federation

References

  1. Utz S.R., Dovganskiy S.I., Odoevskaya O.D. Ispolzovanie metoda poverchnostnoy biopsii kogi v dermatologicheskoy practike. Vestn dermatol venerol 1992; 7: 37-38.
  2. Utz S.R., Dovganskiy S.I., Odoevskaya O.D. Exfoliativnaya kariometriya epidermisa pri psoriase. Vestn dermatol venerol 1995; 5: 4-8.
  3. Weigmann H.J., Lademanna J., Mefferta H. et al. Determination of the Horny Layer Profile by Tape Stripping in Combination with Optical Spectroscopy in the Visible Range as a Prerequisite to Quantify Percutaneous Absorption. Skin Pharmacol Appl Skin Physiol 1999; 12: 34-45.
  4. Lindemann U., Weigmann H.J., Schaefer H. et al. Evaluation of the Pseudo-Absorption Method to Quantify Human Stratumcorneum Removed by Tape Stripping Using Protein Absorption. Skin Pharmacol Appl Skin Physiol 2003; 16: 228-236.
  5. Dreher F., Modjtahedi B.S., Modjtahedi S.P. et al. Quantification of stratum corneum removal by adhesive tape stripping by total protein assay in 96-well microplates. Skin Res and Tech 2005; 11: 97-101.
  6. Van der Molen R.G., Spies F., Van ‘t Noordende J. M. et al. Tape stripping of human stratum corneum yields cell layers that originate from various depths because of furrows in the skin. Arch Dermatol Res 1997; 289: 514-518.
  7. Weigmann H.J., Lindemanna U., Antonioub C. et al. UV/VIS Absorbance Allows Rapid, Accurate, and Reproducible Mass Determination of Corneocytes Removed by Tape Stripping Skin. Pharmacol Appl Skin Physiol 2003; 16: 217-227.
  8. Galanzha E.I., Tuchin V.V., Luo Q.,Cheng H. et al. The action of osmotically active drugs on optical properties of skin and state of microcirculation in experiments. Asian J Phys 2001; 104: 503-511.
  9. Zimnyakov D.A., Sinichkin V.P., Ushakova O.V. Opticheskaya anizotropiya fibroznich biotkaney: analis vliyaniya strukturnich characteristic. Kvantovaya electronika 2007; 37: (8) 777- 783.
  10. Kuzmin V.A., Meglinskiy I.V. Obratnoe rasseyanie sveta s lineynoy I krugovoy polyarizaciyami v sluchayno-neodnorodnich sredach. Optica I spectroskopiya 2009; 106: (2): 294- 305
  11. Jacobi U., Waibler E., Sterry W. et al. In vivo determination of the long-term reservoir of the horny layer using laser scanning microscopy. Laser Phys 2005; 15: (4): 565-569.
  12. Eppich E., Beuthan J., Dressier C. et al. Optical phase measurements on biological cells. Laser Phys 2000; 10: 467-477.
  13. Wang L.V., Wu H.I. Biomedical Optics. ISBN 978-0-471-74304-0, 2007.
  14. Wang R.K., Tuchin V.V. Optical tissue clearing to enhance imaging performance for OCT. Optical Coherence Tomography: Technology and Applications. Ed. by W. Drexler, J.G. Fujimoto. Berlin: Springer. 2008; 851-882.
  15. Tuchin V.V., Zimnyakov D.A., Maksimova I.L. et al. The coherent, low-coherent and polarized light interraction with tissues undergo the refractive indices matching control. Proc. SPIE 1998; 3251: 12-21.
  16. Guo X., Gou Z., Wei H. et al. In vivo comparison of the potical clearing efficacy of optical clearing agents in human skin by quantifying permeability using optical coherence tomography. Photochem Photobiol 2011; 87: (3): 734-740.
  17. Wen X., Jacques S.L., Tuchin V.V. et al. Enhanced optical clearing of skin in vivo and OCT in-depth imaging. J Biomed Opt 2012; 17: (6): 22-66.
  18. Tuchin V.V. Laseri I volokonnaya optica v biomedicinskich issledovaniyach. Saratov: izd-vo Saratovskogo un-ta 1998; (383): 3. [Тучин В.В. Лазеры и волоконная оптика в биомедицинских исследованиях. Саратов: изд-во Саратовского ун-та 1998; (383): 3.]
  19. Thilo Gambichler, Georg Moussa, Michael Sand et al. Applications of optical coherence tomography in dermatology. Journal of Dermatological Science 2005; 40: 85-94.
  20. Maria Ziolkowskaa, Carsten M.Philippa, Julia Liebscherb, Hans-Peter Berliena. OCT of healthy skin, actinic skin and NMSC lesions. Received 12 July 2009.
  21. Tuchin V.V., Zimnyakov D.A.,Maksimova I.L. et al. The coherent, low-coherent and polarized light interraction with tissues undergo the refractive indices matching control. Proc SPIE 1998; 3251: 12-21.
  22. Genina E.A., Bashkatov A.N., Sinichkin U.P. et al. Opticheskoe prosvetlenie kogi pod deystviem glicerina: issledovanie ex vivo i in vitro. Opt spect 2010; 109: (2): 256-263.
  23. Chernova S.P., Kuznetsova N.V., Pravdin A.B. et al. Dynamics of optical clearing of human skin in vivo. Proc SPIE 2000; 4162: 227-235.
  24. Dermatologiya Fiztparicka v klinincheskoy ptactike: Vt. / Klaus Volf, Louell A. Goldsmeet, Stiven I. Kaz et. al.; obsh. red. akad. A.A. Kubanovoy. M.: Izd-vo Panfilova; BINOM. Laboratoriya znaniy 2012; 1: 46-52.
  25. Kolichestvennie metodi ocenki intensivnosti deskvamacii pri izuchenii effectivnosti antipsoriaticheskoy terapii. Vestn dermat venerol 1997; 2: 4-7.
  26. Socolova T.V. Chesotka. Novoe v etiologii, epidemiologii, klinike, diagnostike I profilactike (lekciya). Ros g kog ven bol 2001; 1: 27-39.
  27. Rufli Th., Mumcuoglu Y. The hair follicle mites Demodex folliculorum and Demodex brevis: biology and medical importance. Dermatology 1981; 162: 1-11.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2015 Utz S.R., Zimnyakov D.A., Galkina E.M., Reshetnikova E.M.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 60448 от 30.12.2014.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies