Targeted photosensitizer delivery: A prospective approach to vitiligo photochemotherapy

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Abstract

Aim. In this work, the authors set out to develop an effective method for the intrafollicular delivery of “Ammi majus fructuum furocumarines” photosensitizer (AMFF) followed by UVA irradiation (λ = 320–400 nm). Materials and methods. The proposed delivery method consists in using calcium carbonate particles acting as AMFF carriers. In vivo monitoring of hair follicle filling was carried out via optical coherence tomography, as well as by means of analyzing epilated hair using confocal laser scanning microscopy. Following the administration of free and encapsulated AMFF to three healthy volunteers, the character of UVA-induced skin pigmentation was registered under dermatoscopic examination. Results. The obtained results demonstrate a profuse filling of hair follicles with calcium carbonate particles, thus confirming the possibility of intrafollicular photosensitizer delivery. It was established that exposure to UVA irradiation causes intense pigment accumulation in the area of AMFF carrier administration. Conclusion. The proposed method of the targeted photosensitizer delivery allows photochemical therapy to be improved.

About the authors

S. R. Utz

Saratov State Medical University named after V. I. Razumovsky, Ministry of Health of the Russian Federation

Email: fake@neicon.ru
Dr. Sci. (Med.), Prof., Departmental Head, Department of Skin and Venereal Diseases Россия

G. B. Sukhorukov

Queen Mary University of London

Email: fake@neicon.ru
Cand. Sci. (Phys.-Math.), Prof., Chair of Biomedical Materials Division, School of Engineering and Materials Science Великобритания

V. V. Tuchin

Saratov State University;
National Research Tomsk State University

Email: fake@neicon.ru
Dr. Sci. (Phys.-Math.), Prof., Departmental Head, Department of Optics and Biophotonics, Director of Research and Educational Institute of Optics and Biophotonics, Saratov State University; Research Supervisor, Laboratory of Biophotonics, National Research Tomsk State University Россия

D. A. Gorin

Saratov State University

Email: fake@neicon.ru
Dr. Sci. (Chem.) Associate Professor, Deputy Director of Research and Educational Institute of Optics and Biophotonics; Prof., Department of Semiconductor Physics Россия

E. A. Genina

Saratov State University;
National Research Tomsk State University

Email: fake@neicon.ru
Cand. Sci. (Phys.-Math.), Associate Professor, Department of Optics and Biophotonics, Senior Researcher, Laboratory of Biomedical Optics, Research and Educational Institute of Optics and Biophotonics, Saratov State University; Leading Researcher, Laboratory of Biophotonics, National Research Tomsk State University Россия

Yu. I. Svenskaya

Saratov State University

Author for correspondence.
Email: yulia_svenskaya@mail.ru
Cand. Sci. (Phys.-Math.), Senior Researcher, Laboratory of Remote Controlled Theranostic Systems Россия

E. E. Talnikova

Saratov State Medical University named after V. I. Razumovsky, Ministry of Health of the Russian Federation

Email: fake@neicon.ru
Registrar, Department of Skin and Venereal Diseases Россия

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Copyright (c) 2019 Utz S.R., Sukhorukov G.B., Tuchin V.V., Gorin D.A., Genina E.A., Svenskaya Y.I., Talnikova E.E.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
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