Using the Line Blot Syphilis test system for diagnosingsyphilis by the linear immunoblotting method


ZAO ECOlab (Russia) developed Line Blot Syphilis, a new test system on the basis of the linear immunoblotting method
using Treponema рallidum recombinant antigens. The article assessed the diagnostic value of the Line Blot Syphilis test
system in the form of a confirmatory test. As a part of the conducted study, the test system demonstrated its absolute
sensitivity and specificity to serum-positive (n = 237) and serum-negative (n = 114) samples, in which the presence
or absence of Т.pallidum antibodies was confirmed by two treponemal tests. As a result of examining 14 samples
attributed to doubtful analytes based on two test results (passive hemagglutination test and immunofluorescence test
with absorption), the data compliance between the Line Blot Syphilis test system and data from INNO-LIA Syphilis Score
amounted to 100% (14/14) or 93% (13/14) for the immune-enzyme assay and 57% (8/14) for the immunofluorescence
test with absorption. It is recommended to use the Line Blot Syphilis test system (ZAO ECOlab) as a confirmatory syphilis


  1. Larsen S.A., Steiner B.M., Rudolph A.H. Laboratory diagnosis and interpretation of tests for syphilis. Clin Microbiol Rev 1995; 8: 1-21.
  2. Wheeler H.L., Agarwal S., Goh B.T. Dark ground microscopy and treponemal serological tests in the diagnosis of early syphilis. Sex Transm Infect 2004; 80: 411-414.
  3. Morse S.A. Advances in diagnostic tests for bacterial STDs. Salud Publica Mex 2003; 45 Suppl 5: 698-708.
  4. Rusnak J.M., Butzin C., MacGlason D. et al. False-positive rapid plasma reagin tests in human immunodeficiency virus infection and relationship to anticardiolipin antibody and serum immunoglobulin levels. J Infect Dis 1994; 169: 1356-1359.
  5. Nandwani R., Evans D.T.P. Are you sure it's syphilis? A review of false positive serology. Int J STD AIDS 1995; 6: 241-248.
  6. Кубанова А.А., Говорун В.М., Китаева Н.В. и др. Достижения и перспективы изучения Treponema pallidum. Вестн. дерматол., 2006; 5: 34-37.
  7. Carlsson B., Hanson H.S., Wasserman J. et al. Evaluation of the fluorescent treponemal antibody-absorption (FTA-ABS) test specificity. Acta Dermatol Venereol 1991; 71: 306-311.
  8. Dettori G., Grillo R., Mora G. et al. Evaluation of Western immunoblotting technique in the serological diagnosis of human syphilitic infections. Eur J Epidemiol 1989; 5: 22-30.
  9. Byrne R.E., Laska S., Bell M. et al. Evaluation of a Treponema pallidum Western immunoblot assay as a confirmatory test for syphilis. J Clin Microbiol 1992; 30: 115-122.
  10. Ebel A., Vanneste L., Cardinaels M. et al. Validation of the INNO-LIA syphilis kit as a confirmatory assay for Treponema pallidum antibodies. J Clin Microbiol 2000; 38: 215-219.
  11. Norris S.J., and the Treponema pallidum Polypeptide Research Group. Polypeptides of Treponema pallidum: progress toward understanding their structural, functional, and immunologic roles. Microbiol Rev 1993; 57: 750-779.
  12. Sambri V., Marangoni A., Eyer C. et al. Western Immunoblotting with Five Treponema pallidum Recombinant Antigens for Serologic Diagnosis of Syphilis. Clin Diagnost Lab Immunol 2001; 8: 534-539.
  13. Hagedorn H.J., Kraminer-Hagedorn A., De Bosschere K et al. Evaluation of INNO-LIA syphilis assay as a confirmatory test for syphilis. J Clin Microbiol 2002; 40: 973-978.
  14. Фриго Н.В., Ротонов С.В., Нестеренко В.Г. и др. Результаты изучения диагностической эффективности новой тест-системы линей- ного иммуноферментного анализа «Inno- Lia™ Syphilis Score». Клин. лаб. диагн., 2006; 3: 36-41.
  15. Кубанова А.А., Фриго Н.В., Ротанов С.В. и др. Иммуноблотинг в диагностике ранних форм сифилиса. Вестн. дерматол., 2006; 2: 4-11.
  16. Фриго Н.В., Ротанов С.В., Дударева Л.А. и др. Опыт использования метода иммуно- блотинга для диагностики сифилиса. Вестн. дерматол., 2008; 4: 57-62.
  17. Schmidt B.L., Edjlalipour M., Luger A. Comparative evaluation of nine different enzyme-linked immunosorbent assays for determination of antibodies against Treponema pallidum in patients with primary syphilis. J Clin Microbiol 2000; 38: 1279-82.
  18. Zrein M., Maure I., Boursier F. et al. Recombinant antigen-based enzyme immunoassay for screening of Treponema pallidum antibodies in blood bank routine. J Clin Microbiol 1995; 33: 525-7.
  19. Young H., Moyes A., Seagar L. et al. Novel recombinant-antigen enzyme immunoassay for serological diagnosis of syphilis. J Clin Microbiol 1998; 36: 913-7.
  20. Sordillo E.M., Hoehl B., and Belch J. Falsenegative fluorescent treponemal tests and confirmation of syphilis infection.1998; J. Infect. Dis. 178: 294-295.
  21. Кубанова А.А., Нестеренко В.Г., Фриго Н.В. и др. Опыт использования метода иммуно-блотинга для диагностики сифилиса. Вестн. дерматол., 2006; 2: 4-11.
  22. Кубанова А.А., Фриго Н.В., Ротанов С.В., Нестеренко В.Г., Ловенецкий А.Н., Дударева Л.А. Опыт использования метода иммуноблоттинга для диагностики сифилиса. Вестник дерматологии и венерологии 2006. (2): 4-11.

Copyright (c) 2011 Mardanly C.G., Arsen'eva V.A., Frigo N.V., Rotanov S.V., Amelina E.A., Zakharov M.V., Mardanly S.G., Arseniyeva V.A., Frigo N.V., Rotanov S.V., Amelina Y.A., Zakharov M.V.

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