Immunohistochemistry method and diagnostics of mycosis fungoides

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Abstract

Clinical patient examinations are not enough for diagnosing lymphoproliferative skin diseases now. Histological diagnostics is an important auxiliary method; however, it is not always applicable. PCR is not a universal method for determining the clonality of infiltrate cells. The article describes present-day diagnostics methods with an emphasis on the immunohistochemistry method making it possible to identify the cell composition of affected skin.

About the authors

A. S. Zhukov

Military Medical Academy named after S.M. Kirov Ministry of Defense of the Russian Federation

Author for correspondence.
Email: md.zhukov@gmail.com
Russian Federation

I. E. Belousova

Military Medical Academy named after S.M. Kirov Ministry of Defense of the Russian Federation

Email: noemail@neicon.ru
Russian Federation

A. V. Samtsov

Military Medical Academy named after S.M. Kirov Ministry of Defense of the Russian Federation

Email: noemail@neicon.ru
Russian Federation

References

  1. Wilson L.D., Hinds G.A., Yu J.B. Age, race, sex, stage, and incidence of cutaneous lymphoma. Clin Lymphoma Myeloma Leuk 2012 Oct; 12 (5): 291-6.
  2. Weinstock M.A., Horm J.W. Mycosis fungoides in the United States. Increasing incidence and descriptive epidemiology. JAMA 1988 Jul 1; 260 (1): 42-6.
  3. Lezvinskaja E.M., Molochkov V.A., Larina N.K. Condition of malignant lymphoma of the skin in the Moscow region and ways to improve the treatment and diagnostic care to patients. Ros zhurn kozh i ven bol 2000; 4: 12-17. [Лезвинская Е.М., Молочков В.А., Ларина Н.К. Состояние заболеваемости злокачественными лимфомами кожи в Московской области и пути совершенствования лечебно-диагностической помощи больным. Росс журн кож и вен бол 2000; (4): 12-17.]
  4. Kungurov N.V., Kohan M.M., Kuklin I.A., Rimar O.G. On improving the delivery of specialized medical care for patients with malignant lymphomas of the skin. Vestn dermatol i venerol 2010; 3: 4-11. [Кунгуров Н.В., Кохан М.М., Куклин И.А., Римар О.Г. О совершенствовании оказания специализированной медицинской помощи больным злокачественными лимфомами кожи. Вестн дерматол и венерол, ФГУ «Уральский научноисследовательский институт дерматовенерологии и иммунопатологии Росмедтехнологий» 2010; (3): 4-11.]
  5. Pimpinelli N., Olsen E.A., Santucci M. et al. Defining early mycosis fungoides. J Am Acad Dermatol 2005; 53: 1053-1063.
  6. Burg G., Kempf W., Haeffner A.C., Nestle F.O., Hess Schmid M., Doebbeling U., Mueller B., Dummer R. Cutaneous lymphomas. Curr Probl Dermatol 1997; 9: 137-204.
  7. Zackheim H.S., McCalmont T.H. Mycosis fungoides: the great imitator. J Am Acad Dermatol 2002 Dec; 47 (6): 914-8.
  8. Kazakov D.V., Burg G., Kempf W. Clinicopatho-logical spectrum of mycosis fungoides. J Eur Acad Dermatol Venereol 2004 Jul; 18 (4): 397-415.
  9. Nashan D., Faulhaber D., Ständer S., Luger T.A., Stadler R. Mycosis fungoides: a dermatological masquerader. Br J Dermatol 2007 Jan; 156 (1): 1-10.
  10. Belousova I.E., Kazakov D.V., Krivolapov Ju.A. Modern approaches to the diagnosis and treatment of primary cutaneous lymphomas based on the new WHO/EORTS-classification. T-cell lymphoma of the skin. Arhiv patologii 2007; 69 (5): 11-17. [Белоусова И.Э., Казаков Д.В., Криволапов Ю.А. Современные подходы к диагностике и лечению первичных лимфом кожи на основе новой ВОЗ/ ЕОРТС-классификации. Т-клеточные лимфомы кожи. Архив патологии 2007; 69 (5): 11-17.]
  11. Bakels V., van Oostveen J.W., van der Putte S.C., Meijer C.J., Willemze R. Immunophenotyping and gene rearrangement analysis provide additional criteria to differentiate between cutaneous T-cell lymphomas and pseudo-T-cell lymphomas. Am J Pathol 1997; 150: 1941-9.
  12. Bergman R., Faclieru D., Sahar D., Sander C.A., Kerner H., Ben-Aryeh Y. et al. Immunophenotyping and T-cell receptor gamma gene rearrangement analysis as an adjunct to the histopathologic diagnosis of mycosis fungoides. J Am Acad Dermatol 1998; 39: 554-9.
  13. Olsen E., Vonderheid E., Pimpinelli N., Willemze R., Kim Y., Knobler R., Zackheim H., Duvic M., Es-trach T., Lamberg S., Wood G., Dummer R., Ranki A., Burg G., Heald P., Pittelkow M., Bernengo M.G., Sterry W., Laroche L., Trautinger F., Whittaker S.; ISCL/EORTC. Revisions to the staging and classification of mycosis fungoides and Sezary syndrome: a proposal of the International Society for Cutaneous Lymphomas (ISCL) and the cutaneous lymphoma task force of the European Organization ofResearch and Treatment of Cancer (EORTC). Blood 2007 Sep 15; 110 (6): 1713-22.
  14. Burg G., Kempf W. Cutaneous Lymphomas (Basic and Clinical Dermatology) Informa Health-care 2005; 592.
  15. Berger C.L., Hanlon D., Kanada D., Dhodapkar M., Lombillo V., Wang N., Christensen I., Howe G., Crouch J., El-Fishawy P., Edelson R. The growth of cutaneous T-cell lymphoma is stimulated by immature dendritic cells. Blood 2002 Apr 15; 99 (8): 2929-39.
  16. Tirumalae R., Panjwani P.K. Origin Use of CD4, CD8, and CD1a Immunostains in Distinguishing Mycosis Fungoides from its Inflammatory Mimics: A Pilot Study.Indian J Dermatol 2012 Nov; 57 (6): 424-7.
  17. Saint-Vis B., Vincent J., Vandenabeele S., Vanbervliet B., Pin J.J., Aït-Yahia S., Patel S., Mattei M.G., Banchereau J., Zurawski S., Davoust J., Caux C., Lebecque S. A novel lysosome-associated membrane glycoprotein, DC-LAMP, induced upon DC maturation, is transiently expressed in MHC class II compartment. Immunity 1998 Sep; 9 (3): 325-36.
  18. Prechtel A.T., Steinkasserer A. CD83: an update on functions and prospects of the maturation marker of dendritic cells. Arch Dermatol Res 2007 May; 299 (2): 59-69.
  19. McMillan E.M., Beeman K., Wasik R., Everett M.A. Demonstration of OKT 6-reactive cells in mycosis fungoides. J Am AcadDermatol 1982; 6: 880-887.
  20. Goteri G., Filosa A., Mannello B., Stramazzotti D., Rupoli S., Leoni P., Fabris G. Density of neoplastic lymphoid infiltrate, CD8+ T-cells, and CD1a+ dendritic cells in mycosis fungoides. J Clin Pathol 2003 Jun; 56 (6): 453-8.
  21. Pigozzi B., Bordignon M., BelloniFortina A. et al. Expression of the CD1a molecule in B- and T-lymphoproliferative skin conditions.Oncol Rep 2006; 15: 347-351.
  22. Meissner K., Loning T., Rehpenning W. Epidermal Langerinerhans cells and prognosis of patients with mycosis fungoides and Se'zary syndrome. In Vivo 1993; 7: 277-280.
  23. Der-Petrossian M., Valencak J., Jonak C., Klosner G., Dani T., Müllauer L., Pehamberger H., Knobler R., Trautinger F. Dermal infiltrates of cutaneous T-cell lymphomas with epidermotropism but not other cutaneous lymphomas are abundant with langerinerin dendritic cells. J Eur Acad Dermatol Venereol. 2011 Aug; 25 (8): 922-7.
  24. Schlapbach C., Ochsenbein A., Kaelin U., Hassan A.S., Hunger R.E., Yawalkar N. High numbers of DC-SIGN+ dendritic cells in lesional skin of cutaneous T-cell lymphoma. J Am Acad Dermatol 2010 Jun; 62 (6): 995-1004.
  25. Schwingshackl P., Obermoser G., Nguyen V.A., Fritsch P., Sepp N., Romani N. Distribu-tion and maturation of skin dendritic cell subsets in two forms of cutaneous T-cell lymphoma: mycosis fungoides and Sézary syndrome. Acta Derm Venereol 2012 May; 92 (3): 269-75.
  26. Zhukov A.S., Belousova I.E., Khairutdinov V.R., Samtcov A.V. Role of langerin+ and CD83+ DC in pathogenesis of mycosis fungoides. Vestn dermatol i venerol 2013; 4: 38-43. [Жуков А.С., Белоусова И.Э., Хайрутдинов В.Р., Самцов А.В. Роль лангеринпозитивных и CD83+ клеток в патогенезе грибовидного микоза. Вестн дерматол и венерол 2013; (4): 38-43.]
  27. Reinhold U., Pawelec G., Fratila A., Leippold S., Bauer R., Kreysel H.W. Phenotypic and functional characterization of tumor infiltrating lymphocytes in mycosis fungoides: continuous growth of CD4+ CD45R+ T-cell clones with suppressor-inducer activity. J Invest Dermatol 1990 Mar; 94 (3): 304-9.
  28. Kasprzycka M., Zhang Q., Witkiewicz A., Marzec M., Potoczek M., Liu X. et al. Gamma c-signaling cytokines induce a regulatory T cell phenotype in malignant CD4+ T lymphocytes. J Immunol 2008; 181: 2506-2512.
  29. Fried I., Cerroni L. FOXP3 in sequential biopsies of progressive mycosis fungoides. Am J Dermatopa-thol 2012 May; 34 (3): 263-5.
  30. Babichenko I.I., Kovjazin V.A. New methods of im-munohistochemical diagnosis of tumor growth: Textbook. M: RUDN 2008; 109. [Бабиченко И.И., Ковязин В.А. Новые методы иммуногистохимической диагностики опухолевого роста: Учеб. пособие. М: РУДН 2008; 109.]
  31. Jovanovioh M.P., Jakovich L., Bogdanovich A., Markovich O., Martinovich V.C., Mihaljevich B. Poor outcome in patients with diffuse large B-cell lymphoma is associated with high percentage of bcl-2 and Ki 67-positive tumor cells. Vojnosanit Pregl 2009 Sep; 66 (9): 738-43.
  32. Dummer R., Michie S.A., Kell D., Gould J.W., Haef-fner A.C., Smoller B.R., Warnke R.A.,Wood G.S. Expression of bcl-2 protein and Ki-67 nuclear proliferation antigen in benign and malignant cutaneous T-cell infiltrates. J Cutan Pathol 1995 Feb; 22 (1): 11-7.
  33. Gambichler T., Bischoff S., Bechara F.G., Altmeyer P., Kreuter A. Expression of proliferation markers and cell cycle regulators in T cell lymphoproliferative skin disorders. J Dermatol Sci 2008 Feb; 49 (2): 125-32.
  34. Agnarsson B.A., Vonderheid E.C., Kadin M.E. Cutaneous T cell lymphoma with suppressor-cytotoxic (CD8) phenotype: identification of rapidly progressive and chronic subtypes. J Am AcadDermatol 1990; 22: 569-577.

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Copyright (c) 2014 Zhukov A.S., Belousova I.E., Samtsov A.V.

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