Potential of regenerative medicine for treatment of vitiligo patients

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The article presents a review of publishes sources on the efficacy of methods such as tissue engineering and cellular transplantation of autologous melanocytes for treatment of vitiligo patients. The article describes general principles of treatment and particular features of current melanocyte transplantation methods.

About the authors

A. A. Kubanova

State Research Center of Dermatovenereology and Cosmetology, Ministry of healthcare of the Russian Federation

Author for correspondence.
Email: noemail@neicon.ru
Russian Federation

V. A. Volnukhin

State Research Center of Dermatovenereology and Cosmetology, Ministry of healthcare of the Russian Federation

Email: volnuhin@cnikvi.ru
Russian Federation

D. V. Proshutinskaya

State Research Center of Dermatovenereology and Cosmetology, Ministry of healthcare of the Russian Federation

Email: noemail@neicon.ru
Russian Federation

M. B. Zhilova

State Research Center of Dermatovenereology and Cosmetology, Ministry of healthcare of the Russian Federation

Email: noemail@neicon.ru
Russian Federation

V. V. Chikin

State Research Center of Dermatovenereology and Cosmetology, Ministry of healthcare of the Russian Federation

Email: noemail@neicon.ru
Russian Federation

A. E. Karamova

State Research Center of Dermatovenereology and Cosmetology, Ministry of healthcare of the Russian Federation

Email: noemail@neicon.ru
Russian Federation

R. R. Saitburkhanov

State Research Center of Dermatovenereology and Cosmetology, Ministry of healthcare of the Russian Federation

Email: noemail@neicon.ru
Russian Federation


  1. Mason C, Dunnill P: A brief definition of regenerative medicine. Regen. Med. 2008; 3 (1), 1-5.
  2. Mason C, Manzotti E: Regen: the industry responsible for cell-based therapies. Regen. Med. 2009; 4 (6), 783-785.
  3. Lewin ML, Peck SM. Pigment studies in skin grafts on experimental animals. J In-vest Dermat 1941; 4: 483-503.
  4. Spencer GA, Tolmach JA. Exchange grafts in vitiligo. J Invest Dermatol. 1952; 19 (1): 1-5.
  5. Behl PN. Treatment of vitiligo with homologous thin Thiersch skin grafts. Curr Med Pract 1964; 8: 218- 221 (Цит. по Surgical management of vitiligo / ed. by S. Gupta et al. 2007 Blackwell Publishing Ltd Massachusetts, Oxford, Victoria.)
  6. Falabella R. Epidermal grafting. An original technique and its application in achro-mic and granulating areas. Arch Dermatol 1971; 104 (6): 592-600.
  7. Falabella R. Repigmentation of segmental vitiligo by autologous minigrafting. J Am Acad Dermatol 1983; 9 (4): 514-521.
  8. Falabella R, Borrero I, Escobar C. [In vitro culture of melanocyte-bearing epidermis and its application in the treatment of vitiligo and the stable leukodermas]. Med Cu-tan Ibero Lat Am. 1989; 17 (3): 193-198.
  9. Gauthier Y, Surleve-Bazeille JE. Autologous grafting with noncultured melano-cytes: a simplified method for treatment of depigmented lesions. J Am Acad Derma-tol 1992; 26 (2 Pt 1): 191-194.
  10. Olsson MJ, Juhlin L. Leucoderma treated by transplantation of a basal cell layer en-riched suspension. Br J Dermatol 1998; 138 (4): 644-648.
  11. Olsson MJ, Juhlin L. Melanocyte transplantation in vitiligo. Lancet 1992 17; 340 (8825): 981.
  12. Na GY, Seo SK, Choi SK. Single hair grafting for the treatment of vitiligo. J Am Acad Dermatol. 1998; 38 (4): 580-584.
  13. Vanscheidt W, Hunziker T. Repigmentation by outer-root-sheath-derived melano-cytes: proof of concept in vitiligo and leucoderma. Dermatology 2009; 218 (4): 342-343.
  14. Thomas A.J, Erickson C.A. The making of a melanocyte: the specification of melanoblasts from the neural crest. Pigment Cell Melanoma Res 2008; 21: 598-610.
  15. Erickson, C.A., and Reedy, M.V. Neural crest development: the interplay between morphogenesis and cell differentiation. Curr. Top. Dev. Biol. 1998; 40, 177-209.
  16. Dupin E, Le Douarin N.M. Development of melanocyte precursors from the verte-brate neural crest. Oncogene 2003; 22: 3016-3023.
  17. White RM, Zon LI. Melanocytes in development, regeneration, and cancer. Cell Stem Cell 2008; 3: 242-252.
  18. Baynash, A.G., Hosoda, K., Giaid, A., Richardson, J.A., Emoto, N., Hammer, R.E., and Yanagisawa, M. Interaction of endothelin-3 with endothelin- B receptor is es-sential for development of epidermal melanocytes and enteric neurons. Cell 1994; 79: 1277-1285.
  19. Lee, H.O., Levorse, J.M., and Shin, M.K. The endothelin receptor-B is required for the migration of neural crest-derived melanocyte and enteric neuron precursors. Dev. Biol. 2003; 259: 162-175.
  20. Widlund H.R, Fisher D.E. Microphthalamia-associated transcription factor: a criti-cal regulator of pigment cell development and survival. Oncogene 2003; 22: 3035-3041.
  21. Grichnik J.M. Kit and melanocyte migration. J Invest Dermatol 2006; 126: 945-947.
  22. Giebel L.B. and Spritz R.A. Mutation of the KIT (mast/stem cell growth factor re-ceptor) protooncogene in human piebaldism. Proc. Natl. Acad. Sci. USA 1991; 88: 8696-8699.
  23. Hirobe T. Role of keratinocyte-derived factors involved in regulating the prolifera-tion and differentiation of mammalian epidermal melanocytes. Pigment Cell Res 2005; 18: 2-12.
  24. Real C, Glavieux-Pardanaud C, Le Douarin NM, Dupin E. Clonally cultured differ-entiated pigment cells can dedifferentiate and generate multipotent progenitors with self-renewing potential. Dev Biol 2006; 300: 656-669.
  25. Kawaguchi Y, Mori N, Nakayama A. Kit(+) melanocytes seem to contribute to melanocyte proliferation after UV exposure as precursor cells. J Invest Dermatol 2001; 116: 920-925.
  26. Grichnik JM, Ali WN, Burch JA, et al. KIT expression reveals a population of pre-cursor melanocytes in human skin. J Invest Dermatol 1996; 106: 967-971.
  27. Tsukamoto K, Osada A, Kitamura R, Ohkouchi M, Shimada S, Takayama O. Ap-proaches to repigmentation of vitiligo skin: new treatment with ultrasonic abrasion, seed-grafting and psoralen plus ultraviolet A therapy. Pigment Cell Res. 2002; 15 (5): 331-334.
  28. Brysk MM1, Newton RC, Rajaraman S et al. Repigmentation of vitiliginous skin by cultured cells. Pigment Cell Res. 1989; 2 (3): 202-7.
  29. Andreassi L, Pianigiani E. et al. A new model of epidermal culture for the surgical treatment of vitiligo. Int J Dermatol 1998; 37 (8): 595-598.
  30. к № 3, 2014
  31. Redondo P. et al. Repigmentation of vitiligo by transplantation of autologous melanocyte cells cultured on amniotic membrane British Journal of Dermatology 2008; 158: 1134-1173.
  32. Sacchidanand S., Thakur P., Purohit V., Sujaya S.N. Follicular unit extraction as a therapeutic option for vitiligo. J Cutan Aesthetic Surgery 2013; 6 (4): 229-231.
  33. Pianigiani E, Andreassi A, Andreassi L. Autografts and cultured epidermis in the treatment of vitiligo. Clin Dermatol. 2005; 23 (4): 424-429.
  34. Czajkowski R, Placek W, Drewa T, Kowaliszyn B, Sir J, Weiss W. Autologous cul-tured melanocytes in vitiligo treatment. Dermatol Surg 2007; 33: 1027-36.
  35. Czajkowski R. Comparison of melanocytes transplantation methods for the treat-ment of vitiligo. Dermatol Surg 2004; 30 (11): 1400-1405.
  36. Mulekar SV. Melanocyte-keratinocyte cell transplantation for stable vitiligo.Int J Dermatol 2003; 42: 132-6.
  37. van Geel N, Ongenae K, De Mil M, Haeghen YV, Vervaet C, Naeyaert JM. Double-blind placebocontrolled study of autologous transplanted epidermal cell suspen-sions for repigmenting vitiligo. Arch Dermatol 2004; 140: 1203-1208.
  38. Gauthier Y, Benzekri L. Non-cultured epidermal suspension in vitiligo: from labo-ratory to clinic. Indian J Dermatol Venereol Leprol. 2012; 78 (1): 59-63.
  39. Mulekar S.V. Childhood vitiligo: a long-term study of localized vitiligo treated by noncultured cellular grafting. Pediatric Dermatology 2010; 27; 132-136.
  40. Goh BK, Chua XM, Chong KL, de Mil M, van Geel NA. Simplified cellular graft-ing for treatment of vitiligo and piebaldism: the "6-well plate" technique. Dermatol Surg. 2010 Feb; 36 (2): 203-7.
  41. Falabella R, Barona MI. Update on skin repigmentation therapies in vitiligo. Pig-ment Cell Melanoma Res. 2009; 22 (1): 42-65.
  42. Olsson M.J., Juhlin L. Transplantation of melanocytes in vitiligo. British Journal of Dermatology 1995; 132; 587-591.
  43. Chen YF, Yang PY, Hu DN, Kuo FS, Hung CS, Hung CM. Treatment of vitiligo by transplantation of cultured pure melanocyte suspension: analysis of 120 cases. J Am Acad Dermatol 2004; 51: 68-74.
  44. Mohanty S., Kumar A., Dhawan J., Sreenivas V., Gupta S. Noncultured extracted hair follicle outher root sheath cell suspension for transplantation in vitiligo. British Journal of Dermatology 2011; 164: 1241-1246.
  45. Mulekar SV, Isedeh P. Surgical interventions for vitiligo: an evidence-based review. Br J Dermatol 2013; 169 Suppl 3: 57-66.
  46. Skouge JW, Morison WL, Diwan RV, Rotter S. Autografting and PUVA. A combi-nation therapy for vitiligo. J Dermatol Surg Oncol. 1992; 18 (5): 357-360.
  47. Hann SK, Im S, Bong HW, Park YK. Treatment of stable vitiligo with autologous epidermal grafting and PUVA. J Am Acad Dermatol. 1995; 32 (6): 943-948.
  48. Lahiri K, Malakar S, Sarma N, Banerjee U. Repigmentation of vitiligo with punch grafting and nar-row-band UV-B (311 nm) - a prospective study. Int J Dermatol. 2006; 45 (6): 649-655.
  49. Sheth VM, Currimbhoy SD Feetham HJ et al. Efficacy of narrowband ultraviolet B versus excimer radiation in repigmenting vitiligo after minigrafting on the distal arms. J Am Acad Dermatol. 2012; 67 (2): 318-320.
  50. Wassef C, Lombardi A, Khokher S, Rao BK. Vitiligo surgical, laser, and alternative therapies: a review and case series. J Drugs Dermatol 2013; 12 (6): 685-691.
  51. Back C, Dearman B, Li A, Neild T, Greenwood JE. Noncultured keratino-cyte/melanocyte cosuspension: effect on reepithelialization and repigmentation - a randomized, placebo-controlled study. J Burn Care Res 2009; 30 (3): 408-416.
  52. Felsten LM, Alikhan A, Petronic-Rosic V. Vitiligo: a comprehensive overview Part II: treatment options and approach to treatment. J Am Acad Dermatol. 2011; 65 (3): 493-514.
  53. Krasagakis K, Garbe C, Krüger-Krasagakes S, Orfanos CE. 12-O-tetradecanoylphorbol-13-acetate not only modulates proliferation rates, but also alters antigen expression and LAK-cell susceptibility of normal human melanocytes in vitro. J Invest Dermatol. 1993; 100 (5): 653-659.
  54. van Geel N, Ongenae K, Naeyaert JM. Surgical techniques for vitiligo: A review. Dermatology 2001; 202 (2): 162-166.
  55. Budania A, Parsad D, Kanwar AJ, Dogra S. Comparison between autologousnon-cultured epidermal cell suspension and suction blister epidermal grafting in stable vitiligo: a randomized study. Br J Dermatol 2012; 167 (6): 1295-1301.
  56. Singh C, Parsad D, Kanwar AJ, Dogra S, Kumar R. Comparison between autolo-gous noncultured extracted hair follicle outer root sheath cell suspension and autologous noncultured epidermal cell suspension in the treatment of stable vitiligo: a randomized study. Br J Dermatol 2013; 169 (2): 287-293.
  57. Majid I. Grafting in Vitiligo: How to Get Better Results and How to Avoid Compli-cations. J Cutan Aesthet Surg 2013; 6 (2): 83-89.
  58. Mysore V, Salim T. Cellular grafts in management of leucoderma. Indian J Dermatol 2009; 54 (2): 142-149.



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Copyright (c) 2014 Kubanova A.A., Volnukhin V.A., Proshutinskaya D.V., Zhilova M.B., Chikin V.V., Karamova A.E., Saitburkhanov R.R.

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