Vol 90, No 3 (2014)


Particular features of angiogenesis in lesions in patients suffering from basal cell epithelioma

Khlebnikova A.N., Novoselova N.V.


Basal cell epithelioma is one of the most frequent malignant skin neoplasms. Angiogenesis plays an important part in the development of basal cell epithelioma. The article presents a review of the angiogenesis of this tumor with the help the immunohistochemistry analysis using CD31 and CD34 markers. The authors established a reliable relationship between the number of vessels expressing CD31 and those expressing CD34 in the superficial and nodular forms of the tumor as well as superficial, multi-center, nodular and infiltrative basal cell epitheliomas. A comparison of the number of vessels expressing CD31 and CD34 markers in different histological types made revealed a trend to their growth in the infiltrative type vs. superficial, multi-center and nodular ones.
Vestnik dermatologii i venerologii. 2014;90(3):60-64
pages 60-64 views

Features of matrix metalloproteinase-2 expression in the nuclei of tumor cells of a skin melanoma

Aksenenko M.B., Ruksha T.G.


Goal of the study. To compare expression of matrix metalloproteinase-2 (MMP-2) in the nuclei and cytoplasm of tumor cells and assess the mutual relation between the localization of MMP-2 expression and different clinical and morphologic criteria in patients with a skin melanoma. Materials and methods. Tumor samples obtained from paraffin blocks taken from patients suffering from a skin melanoma and treated at the Krasnoyarsk Territorial Oncologic Dispensary served as the object of the study. Skin tissue samples taken from patients suffering from a skin melanoma (n = 44) were obtained from the Krasnoyarsk Territorial Pathological Anatomy Bureau. Immunohistochemistry was performed based on a standard technique using primary anti-matrix metalloproteinase-2 antibodies. Results. MMP-2 expression was revealed in the nuclei of tumor cells of a skin melanoma in 43.1% of cases and in the cytoplasm in 56.9% of cases. No significant differences in MMP-2 expression in the nuclei and cytoplasm were revealed (p = 0.33). Patients with nuclear expression in tumor cells are characterized by a more favorable prognosis than patients with cytoplasmic enzyme expression in tumor complexes. No dependence of the intracellular localization of MMP-2 expression on the patient sex or age, tumor localization, Clark level of invasion, Breslow’s thickness, particular features of tumor lymphocyte infiltration, tumor growth phase, histological subtype, pigmentation, tumor ulceration and tumor stage according to AJCC (American Joint Committee on Cancer) was revealed. Conclusion. MMP-2 located in the nuclei can be related to hypoxia in the tumor tissue as well as increased peroxynitrite level, which, in its turn, can have an effect on the function and regulation of matrix metalloproteinases. Moreover, MMP-2 with an atypical localization can be related to tumor cells with different biological characteristics within the same tumor, which characterize the intratumoral heterogeneity of neoplasms. The biological and clinical role of changes in the intracellular localization of the enzyme needs further explanation.
Vestnik dermatologii i venerologii. 2014;90(3):65-71
pages 65-71 views

New recombinant T. pallidum antigens Tp0453 and Tp0319 in the diagnostics of syphilis

Runina A.V., Khairullin R.F., Rog K.V., Semina V.I., Rotanov S.V.


A new technology for obtaining a recombinant version of a new dedicated protein T. pallidum Tp0319 for the serological diagnostics of syphilis was gene engineered. As a result, a homogeneous (according to denaturating polyacrylamide gel electrophoresis) recombinant version of T. pallidum Tp0319 protein was obtained. The molecular mass of recombinant T. pallidum Tp0319 protein according to electrophoretic mobility is about 37 kDa, which corresponds to the molecular mass of a fragment of Tp0319 protein without a signal peptide calculated based on the amino-acid sequence (37.3 kDa). According to the developed technology, purified recombinant T. pallidum Tp0319 and Tp0453 proteins were obtained. The proteins were used as antigens as a part of a solid-phase immunoadsorbent for detecting specific IgG to the study protein in the serum of patients suffering from different types of acquired syphilis. The use of the resulting recombinant protein enabled the authors to reveal T. pallidum antibodies in the blood serum in patients suffering from syphilis (primary, secondary, latent early and late stage syphilis). The examination of blood serum samples taken from healthy donors revealed no antibodies. Based on the study results, it is possible to make a conclusion about the possibility to use the resulting recombinant protein (Тр0319) as an extra antigen for diagnostics of syphilis. The introduction of the new antigen in the immunoadsorbent for test systems used for diagnosing syphilis (in the form of immune-enzyme assay, immunoblotting or immune chips) expands the potential of serological diagnostics of this disease due to the expansion of the range of T. pallidum antibodies to be revealed.
Vestnik dermatologii i venerologii. 2014;90(3):72-78
pages 72-78 views

Genetic variants of U. parvum and their role in the development of inflammatory diseases of the urogenital system

Rakhmatullina M.R., Plakhova K.I., Igonina O.N.


The article discusses the results of a study aimed at determining the frequency of different genetic variants of U. parvum in women with inflammatory diseases of the urogenital system and clinically healthy subjects. Molecular typing of 40 samples of U. parvum taken from women with different forms of inflammatory diseases of the urogenital system was performed for the mba gene. The authors revealed a relationship between clinical manifestations of inflammatory diseases of the urogenital system caused by U. parvum and different U. parvum serovars. An infection with 6 U. parvum serovar results in the development of inflammatory processes in the form of cervicitis or vaginitis accompanied by subjective manifestations more frequently than an infection with other serovars under examination (1 and 3) (p < 0.05).
Vestnik dermatologii i venerologii. 2014;90(3):79-84
pages 79-84 views


Actinic reticuloid: сase study

Tlish M.M., Katkhanova O.A., Naatij J.Y., Popovskay E.B., Sicheva N.L.


A rare case study of actinic reticuloid is presented. Analysis of literature, clinical and pathomorphological criteria of I diagnostics are described.
Vestnik dermatologii i venerologii. 2014;90(3):105-109
pages 105-109 views

A case study: progressive recurrent course of a giant condyloma (Buschke - Lowenstein tumor)

Okhlopkov V.A., Naboka M.V., Kosenok V.K., Vodolazsky V.A., Novikov Y.A., Ivasyuk G.N., Glatko S.B.


The authors describe two clinical cases of a malignant giant condyloma (Buschke - Lowenstein tumor), one of which resulted in a lethal outcome. Despite its benign histology, this neoplasm demonstrates evident aggressive and malignant clinical characteristics. The development of this tumor is associated with the papilloma viral infection. Immunodeficiency was diagnosed in the patients in both cases. The lethal outcome was a result of severe anemia, intoxication and local complications. Surgery was the main method of treatment. However, the results were unsatisfactory in view of a weak response to treatment and high risk of relapses. Administration of up-to-date topical immunopotentiators such as Imiquimod seems to be promising because in addition to its principal immunopotentiating effect it may also have a haemostatic action.
Vestnik dermatologii i venerologii. 2014;90(3):110-115
pages 110-115 views


Confocal laser scanning microscopy in vivo for diagnosing melanocytic skin neoplasms

Kubanova A.A., Chikin V.V., Shtirshneider Y.Y., Katunina O.R.


The authors discuss the use of confocal laser scanning microscopy in vivo (CLSM) for diagnosing melanocytic skin neoplasms and its value for early diagnostics of melanoma. CLSM is an innovation noninvasive visual examination method for real-time multiple and painless examinations of the patient’s skin without injuring the skin integument. The method ensures early diagnostics of skin melanomas with high sensitivity and specificity, which makes it possible to use CLSM for screening melanocytic skin neoplasms for the sake of the early onset of treatment to save patient life and health.
Vestnik dermatologii i venerologii. 2014;90(3):85-94
pages 85-94 views

Particular features of the clinical course of the papilloma viral infection depending on quantitative indices of human papilloma viruses of a high carcinogenic risk

Rakhmatulina M.R., Bolshenko N.V., Kuevda D.A., Trofimova O.B.


Goal of the study. To study the dependence of the clinical course of the papilloma viral infection and cytological characteristics of lesions in the cervical mucosa on the quantitative indices of human papilloma virus (HPV). Materials and methods. The study involved 175 female patients with HPV of a high carcinogenic risk including 125 subjects with clinical forms of the papilloma viral infection (PVI) and 50 subjects with subclinical and latent forms of the disease. Laboratory tests were carried out with the use of the polymerase chain reaction including real-time PCR for the quantitative determination of HPV. Cytological examinations of scrapes from the exocervical and endocervical mucosa were carried out according to Leishman I, and the results were interpreted according to Bethesda. Results. The authors established an association between clinical forms of PVI and infection with two or more HPV genotypes, and latent and subclinical forms of the disease and infection with one HPV genotype; HPV Genotype 16 prevails within the structure of HPV of a high carcinogenic risk. It was shown that patients infected with two or more HPV genotypes as well as subjects with subclinical and latent forms of the disease underwent reliably higher viral loads than subjects with anogenital warts and patients infected with one HPV genotype only. A higher HPV viral load was also noted in case of a persisting course of PVI and in patients with high-grade squamous intraepithelial lesions (H-SIL). Conclusion. Women with latent and subclinical forms, persistent PVI course and infected with two or more HPV genotypes of a high carcinogenic risk belong to the high-risk group developing expressed epithelial affections in the cervical mucosa. Quantitative HPV indices exceeding 5 lg of copies of HPV DNA per 100,000 cells belong to unfavorable predictors for the development of intraepithelial affections in the cervical mucosa and stipulate the need to conduct an additional examination (colposcopy or cytology) to exclude their development.
Vestnik dermatologii i venerologii. 2014;90(3):95-104
pages 95-104 views


Potential of regenerative medicine for treatment of vitiligo patients

Kubanova A.A., Volnukhin V.A., Proshutinskaya D.V., Zhilova M.B., Chikin V.V., Karamova A.E., Saitburkhanov R.R.


The article presents a review of publishes sources on the efficacy of methods such as tissue engineering and cellular transplantation of autologous melanocytes for treatment of vitiligo patients. The article describes general principles of treatment and particular features of current melanocyte transplantation methods.
Vestnik dermatologii i venerologii. 2014;90(3):43-52
pages 43-52 views

Revisited diagnostics of hereditary epidermolysis bullosa

Albanova V.I., Chikin V.V., Epishev R.V.


Hereditary epidermolysis bullosa is a big group of hereditary diseases with the main manifestations in the form of blisters on the skin and mucous coat after slight mechanical injuries. It is not always possible to diagnose this disease based on the clinical picture. The article discusses current laboratory diagnostics methods for hereditary epidermolysis bullosa including immunofluorescence antigen mapping (IFM), transmission electron microscopy (TEM) and genetic analysis (molecular or DNA diagnostics) as well as their advantages and disadvantages. TEM determines the micro splitting level and nature of ultrafine changes in the area of the dermoepidermal junction; at the same time, such tests need special expensive equipment. Substantial experience is also needed to analyze the resulting submicroscopic images. IFM determines whether expression of the affected protein related to the disease development is reduced or absent; however, invalid (false positive or false negative) results can be obtained in patients with the reduced expression of the affected protein. Genetic analysis plays a key role for prenatal diagnostics. Therefore, to make an exact diagnosis of hereditary epidermolysis bullosa, it is expedient to apply IFM, TEM and genetic analysis. The need to set an exact diagnosis of the disease is related to the fact that the promising treatment methods being currently developed are aimed at treating patients with certain forms of the disease.
Vestnik dermatologii i venerologii. 2014;90(3):53-59
pages 53-59 views


Resources and activities of dermatovenereological medical organizations in Russian Federation in 2013

Kubanova A.A., Melekhina L.E., Kubanov A.A., Bogdanova E.V.


The article provides analysis of dermatovenereological medical organizations resources and activities and overview of incidence of sexually transmitted infections and skin disorders in Russian Federation in 2013.
Vestnik dermatologii i venerologii. 2014;90(3):16-36
pages 16-36 views

Efficacy of measures aimed at providing specialized medical aid including high-end medical aid in dermatovenerology

Martynov A.A., Vlasova A.V.


Acting on the basis of studying regulatory, legal and reporting documents in the field of high-end medical aid, review of operations of certain federal patient care institutions and results of the authors’ clinical studies, the authors attempted to assess the efficacy of a regular stage in high-end medical aid in the field of dermatovenerology at the expense of the federal budget. The article also provides a brief description of directions for further improvement of high-end medical technologies.
Vestnik dermatologii i venerologii. 2014;90(3):37-42
pages 37-42 views


The benefit of H2 receptors antagonist Rupatadine in treatment for urticaria

Kubanov A.A., Chikin V.V.


Second generation antihistamine drugs are mainly used for the therapy of patients suffering from urticaria; however, they are efficient in 45-60% of cases only. New drugs for treatment of urticaria need to be developed and implemented, and second generation antihistamine drug Rupatadine is one of them. At the same time, Rupatadine efficiently inhibits the inflammatory action of the platelet-activating factor. Due to its double action, Rupatadine used perorally in the dose of 10 mg once a day is an efficient drug for treatment of urticaria, and its safety was confirmed by clinical trials.
Vestnik dermatologii i venerologii. 2014;90(3):116-120
pages 116-120 views

Estimation of clinical efficiency of topical combination therapy patients with pyoderma complicated eczema

Okhlopkov V.A., Zubareva E.Y., Novikov Y.A., Sukach M.S., Repina T.V., Lekavitchus I.Y., Grankina N.V.


The results of direct open-label study of efficacy and portability of combination therapy with topical betamethasone dipropionate and mupirocin are presented. Material and methods. Twenty two patients with pyoderma complicated eczema were treated with ointment for external use (betamethasone dipropionate and mupirocin) 2 times a day for 14 days. Clinical efficacy was assessed by the reliability decrease of index EASI and dermatological symptoms scale index (DSSI). Results. Clinical convalescense was achieved in all patients by the 14th day of therapy. There was a statistically significant decrease in the indices EASI, DSSI. Portability were well. Conclusion. Our study results showed clinical efficacy and portability of topical combination therapy of betamethasone dipropionate and mupirocin in patients with pyoderma complicated eczema.
Vestnik dermatologii i venerologii. 2014;90(3):121-127
pages 121-127 views

Clinical experience of using metronidazole as an external therapy of acne

Statsenko A.V., Bozhchenko A.A., Gorbunov Y.G., Khairutdinov V.R.


Metrogyl gel for external use (metronidazole 1%) has an antioxidant activity and substantially reduces the production of active oxygen compounds by neutrophilic leukocytes in the skin. A combination therapy of light to moderate acne with the use of external retinoids and metronidazole can be more efficient than a monotherapy with the use of these drugs alone. Goal. To assess the clinical efficacy, safety and tolerability of Metrogyl gel for external use in a combination with Klenzit gel (adapalene 0.1%) for treatment of patients with acne vulgaris. Materials and methods. The authors conducted an open-label controlled study of the efficacy, safety and tolerability of Metrogyl gel for external use in a combination with Klenzit gel for external use. As many as 50 patients suffering from acne vulgaris took part in the study, which lasted for eight weeks. The subjects were subdivided into two groups: combination therapy (Group 1, n = 15) and monotherapy with the use of external retinoids (Group 2, n = 15). Results. Adverse effects such as dry skin and peeling were observed among the patients from Group 1 less often (by 3.3 times - 5/35 (14.3%) of cases) than in Group 2 (7/15 (46.7%) of cases) (p = 0.036). The total share of acne patients with clinical recovery and substantial improvement in Group 1 was 33/35 (94.3%), which is 41.4% as much as in Group 2 (10/15 (66.7%)) (p = 0.033). Conclusions. A combination of Metrogyl gel and external retinoids is more efficient than a monotherapy with the use of topical retinoid alone, and such a therapy demonstrates higher tolerability.
Vestnik dermatologii i venerologii. 2014;90(3):128-133
pages 128-133 views

Efficacy of subantimicrobial dose of doxycycline in the treatment of acne of moderate severity: a review of international studie

Khryanin A.A., Reshetnikov O.V.


The article analyzes the recent data concerning the new trends in the treatment of acne vulgaris of moderate severity using tetracyclines. Low-dose regimen of doxycycline is highly effective against inflammatory and noninflammatory lesions in patients with moderate acne on the face with a minority of side effects.
Vestnik dermatologii i venerologii. 2014;90(3):134-139
pages 134-139 views

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Vestnik dermatologii i venerologii. 2014;90(3):140-144
pages 140-144 views

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