Molecular Markers In Forecasting The Clinical Efficacy Of Infliximab In Psoriasis Patients

Abstract


Biosamples (skin tissue samples taken from affection foci and blood serum) from 22 patients suffering from severe to medium psoriasis (8 women and 14 men) aged 19-57 treated with Infliximab were analyzed. As for skin tissue samples, the molecular structure of genes TNF-a, TNF-R-I and TNF-R-II, contents of cytokine TNF-a and its soluble receptors (sTNF-RI and sTNF-RII) and proteome composition was analyzed in skin tissue samples; contents of TNF-a, IL2, IL4, IL6, IL-8 and IL-10 were analyzed in the blood serum. The homozygous TT genotype of TNF-R-II gene at the 676 locus and high IL10 level in the blood serum (>2.7 pg/ml) was associated with the high intensity of the psoriasis patient response to treatment with Infliximab; the homozygous GG genotype of TNF-R-II gene at the 676 locus and low level of IL10 in the blood serum (<1.0 pg/ml) was associated with a weak response or no response to treatment with Infliximab. These results laid the basis for developing a method to forecast the treatment efficacy in psoriasis patients based on a genetically engineered biological drug - Infliximab.
Pilot results indicating a possible mutual relation between the composition of the skin proteome and psoriasis patient's therapeutic response to treatment with Infliximab have been obtained.

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