Particular characteristics of the urogenital tract biota in healthy women of child-bearing potential in real-time PCR studies

The PCR method in the real-time mode helped to analyze the quantitative characteristics of the normal and conditionally pathogenic aerobic/optionally anaerobic, anaerobic biota, mycoplasmas and Candida fungi in the urethra, cervical channel and vagina in healthy women aged 18-45. The analysis revealed absence of any differences between the biotopes by the bacterial spectrum and quantity of both aerobic and anaerobic bacteria with regard to Lactobacillus spp. The biota of all of the women who were younger than 40 and some of the women who were older than 40 had mostly lactobacilli. The microbial composition of the biocenosis in some women older than 40 is characterized by the reduction in the quantity of lactobacilli and their replacement with anaerobic microorganisms/groups of microorganisms, mainly such as Atopobium vaginae, Megasphaera spp./Veillonella spp./Dialister spp., Gardnerella vaginalis/Prevotella bivia/Porphyromonas spp. and Eubacterium spp. Any change in the microbial composition of the urogenital tract biota can serve as a marker of hypoestrogenia.

real-time PCR, biota, urogenital tract, child-bearing potential