Particular characteristics of the urogenital tract biota in healthy women of child-bearing potential in real-time PCR studies

Abstract

The PCR method in the real-time mode helped to analyze the quantitative characteristics of the normal and conditionally pathogenic aerobic/optionally anaerobic, anaerobic biota, mycoplasmas and Candida fungi in the urethra, cervical channel and vagina in healthy women aged 18-45. The analysis revealed absence of any differences between the biotopes by the bacterial spectrum and quantity of both aerobic and anaerobic bacteria with regard to Lactobacillus spp. The biota of all of the women who were younger than 40 and some of the women who were older than 40 had mostly lactobacilli. The microbial composition of the biocenosis in some women older than 40 is characterized by the reduction in the quantity of lactobacilli and their replacement with anaerobic microorganisms/groups of microorganisms, mainly such as Atopobium vaginae, Megasphaera spp./Veillonella spp./Dialister spp., Gardnerella vaginalis/Prevotella bivia/Porphyromonas spp. and Eubacterium spp. Any change in the microbial composition of the urogenital tract biota can serve as a marker of hypoestrogenia.

References

  1. Кубанова А.А., Аковбян В.А., Федоров С.М. и др. Состояние проблемы бактериального вагиноза. Вестник дерматологии и венерологии. 1996; 3:22-26.
  2. Klebanoff M.A., Schwebke J.R., Zhang J. et al. Vulvovaginal symptoms in women with bacterial vaginosis. Obstetric Gynecology. 2004; 104: 267-272.
  3. Hager W.D., McDaniel P.S. Treatment of serious obstetric and gynecologic infections with cefoxitin. Journal of Reproduction Medicine. 1983; 28(5): 337-340.
  4. Spiegel C.A. Bacterial Vaginois. Clinical Microbiology Reviews. 1991; 4(4): 485-502.
  5. Larsen B, Monif G.R.G. Understending the bacterial flora of the female genital tract. Clinical Infections Diseases. 2001; 32:e69-77.
  6. Cauci S., Driussi S., De Santo D. et al. Prevalence of Bacterial Vaginosis and Vaginal Flora Changes in Peri- and Postmenopausal Women. Journal of Clinical Microbiology. 2002; 40(6): 2147-2152.
  7. Li S., Lanuza D., Gulanick M, Penckofer S. et al. Perimenopause: the transition into menopause. Health Care Women Int 1996; 17(4): 293-306.
  8. Mc Kinlay S.M. Menopause. Maturitas 1992; 14:103-115.
  9. Frank O., Bianchi P.G., Campana A.J. The end of fertility: age, fecundity and fecundability in women. Biosocial Scienses. 1994; 26(3): 349-368.
  10. Прилепская В.Н., Царева Н.В. Менопауза: возможности заместительной гормонотерапиии. Русский медицинский журнал. 1998; 8:501-504.
  11. Forsberg J.G. A morphologists approach to the vagina - age- related changes and estrogen sensitivity. Maturitas. 1995; 22(Suppl.22): 7-15.
  12. Сметник В.П., Кулаков В.И. Руководство по климактерию. Москва: Медицинское информационное агентство. 2001.

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Copyright (c) 2010 BOLDYREVA M.N., LIPOVA Y.V., TROFIMOV D.Y., VITVITSKAYA Y.G., GUSKOVA I.A.

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