Vol 89, No 6 (2013)

New regulations for authors.

Extracellular matrix is a system of proteins and polysaccharides maintaining the structural integrity of an organ or tissue. At the same time, in addition to its “classical” function extracellular matrix components regulate many important processes including participation in the signal transmission, regulation of cell division and differentiation, which makes extracellular matrix molecules a prospective target for treatment of a lot of diseases.

Goal. To study polymorphisms in TNFAIP3 (rs610604) and TNIPI (rs17728338) genes in a sample of psoriasis patients and healthy volunteers from the Russian Federation. Materials and methods. By using allele specific hybridization methods in the form of real-time PCR and RFLP assay (Restriction Fragment Length Polymorphism), single-nucleotide polymorphisms of fragments of the following genes associated with predisposition to the development of psoriasis were studied in whole blood samples taken from psoriasis patients (n = 286) and healthy volunteers (n = 89): TNFAIP3 (rs610604) and TNIPI (rs17728338). Results. Psoriasis patients had a reliable increase in the frequency of the A/C heterozygous genotype of rs610604 polymorphism of the TNFAIP3 gene and A allele of rs17728338 polymorphism of the TNIP1 gene (A/G and A/A genotypes), which makes it possible to treat carriers of these genotypes as people with predisposition to the development of psoriasis, and to treat A/C genotypes of rs610604 polymorphism and A/G and A/A genotypes of rs17728338 polymorphism as predictors of the development of psoriasis in the Russian population. Conclusion. these data broaden the perception of genetic factors associated with the development of psoriasis in persons from the Russian population.

The authors disclosed the participation of apoptosis proteins in the development of acantholysis in patients with pemphigus. In this connection, studies of the PERP protein passing apoptosis signals and regulating desmosomal functions in keratinocytes are of interest. There is no information about any studies aimed at the PERP protein expression in patients with pemphigus in available literature. Goal. To assess the PERP protein expression in the skin of patients with pemphigus. Materials and methods. There was a study of 22 patients with pemphigus, a patient with bullous pemphigoid and ten healthy people. The PERP protein expression was studied in the biopsy materials obtained from lesion foci and apparently healthy skin of the patients as well as healthy people using the indirect immunofluorescence method. Results. The PERP protein expression was revealed in patients with pemphigus on areas of apparently intact skin, in lesion foci in the patient with bullous pemphigoid and skin of healthy volunteers in the membrane of keratinocytes from all epidermal layers.The PERP protein expression in the blister operculum in lesion foci in patients with pemphigus was absent. Conclusion. Substantial differences in the PERP protein expression in the blister operculum and apparently intact skin of patients with pemphigus were revealed.

Goal. Production of a recombinant T. pallidum antigen Tp0453 and the evaluation of the efficacy of its clinical use for syphilis diagnosis. Materials and methods. To produce the target recombinant T. pallidum antigen, a Tp0453 protein coding fragment of the DNA of the causative agent of syphilis, was cloned into the pET28а vector. Heterological expression of the recombinant T. pallidum antigen was performed in the E. coli BL21 (DE3) strain. The recombinant Tp0453 six-histidine-tagged protein was purified by the metal-chelate affinity chromatography. The resulting homogenous recombinant T. pallidum Tp0453 protein was used to reveal specific IgG antibodies in the serum of syphilis patients using a microarray technology. Results and conclusion. The use of the resulting recombinant protein enabled authors to reveal anti- T. pallidum antibodies in the blood serum of patients suffering from syphilis (primary, secondary, early and late latent syphilis). None of the uninfected controls had a significant reactivity to the recombinant Tp0453. These data allow to propose, that the recombinant protein Tp0453 show promise for laboratory diagnosis of syphilis. The introduction of Tp0453 antigen into the test systems for diagnosis of syphilis (ELISA, immunoblotting or microarray) increases the potential of serodiagnosis of this disease due to the broader range of the revealed antitreponemal antibodies.

Goal. Comparative assessment of the efficacy of treating certain forms of psoriasis with monochromic ultraviolet light from excimer lamps in a combination with topical glucocorticosteroids or Kartalin cream. Materials and methods. As many as 22 patients (14 male and 8 female aged 25—62) with different clinical forms of psoriasis received a therapy with monochromic ultraviolet light from excimer lamps three times a week. The course of treatment with monochromic ultraviolet light from excimer lamps comprised 6—12 procedures. Topical glucocorticosteroids (GCS) or Kartalin cream were administered externally. Results. Complete regression of eruptions or essential clinical improvement was achieved against the background of treatment in 86% of the patients. At the same time, a clinically significant reduction of psoriatic symptoms was observed in most of the patients treated with Kartalin cream as soon as after the second or third phototherapy procedure while similar results were noted in the patients who received topical glucocorticosteroids only after the fourth procedure. Conclusion. Treatment of psoriatic patients with monochromic ultraviolet light from excimer lamps in a combination with Kartalin cream ensures a fast and positive clinical effect.

The article presents a description of an inherited disease, recessive dystrophic epidermolysis bullosa, in a patient aged 19. The treatment with antibacterial, antiseptic, antianemic and wound-healing drugs, non-adhesive surgical dressings and lowintensive laser light substantially improved the patient’s condition.

The article presents intormation about systemic antibiotics treatment ot acne. The authors discuss issues such as indications tor the administration ot antibiotics, drug selection and combination with external drugs.

The author describes the reasons and frequency of antibiotic resistance of P. acnes in acne patients. He reviews the strategy and methods for doctors to inhibit the prevalence of antibiotic resistance of P. acnes. The article discusses the important role of benzoyl peroxide and synergistic effect of the combination of adapalene and benzoyl peroxide in the finished form to overcome the antibiotic resistance of P. acnes and ensure the treatment efficacy.

A prospective- blind- split-face- comparative study with evaluation of clinical efficacy- tolerability- and safety parameters of two botulinum toxin A (BTA) products (the one containing complexing proteins — Disport®- Ipsen- France/ abobotulinumtoxinA and the one free from complexing proteins — Xeomin®- Merz Pharma- Germany/incobotulinumtoxinA) used for correction of lateral periorbital wrinkles (crow's feet) was performed (at that- electromyographic evaluation of changes in functions of the orbicular muscle of eye was performed for the first time). Materials and Methods. Single injections of both BTA products with equal dose ratio of 3:1 (27 IU Disport® and 9 IU Xeomin®) were performed simultaneously to the right and left side half of the face (orbicular muscles of eye)- respectively- to each of 20 volunteers (women at the age of 40.9) with marked and moderate lateral periorbital wrinkles (2—3 degree according to 4-point Facial Wrinkle Scale/FWS). Clinical and electromyographic assessment was performed 2 weeks- 4 and 6 months after injections. Results. The effect of single dosing of Disport® and Xeomin® did not significantly differ at all assessment time points: both for primary efficacy criterion and for all secondary efficacy criteria; as well as for clinical evaluation by the investigator-subjects’ self-evaluation- and dynamics of M-response parameters for the orbicular muscle of eye. Good tolerability and safety were registered. Conclusion. Study results provided convincing evidence that Disport® and Xeomin® used in accordance with the proposed protocol had a comparable effect and duration of the effect on lateral periotbital wrinkles and the orbicular muscle of eye.